The primary objective of this Core is to provide the physical and intellectual resources for generating genetically engineered mice and rats. In addition to making conventional transgenic mice and rats by pronuclear injection of transgene constructs into single-cell embryos, this core employs cutting edge CRISPR/Cas-mediated genome editing technology for targeted mutations of different kinds directly in zygotes (standard knock-outs, knock-ins, single nucleotide exchanges, generation of LoxP-flanked conditional mutant alleles, large-scale deletions, etc.).
To utilize existing mouse mutants available at centralized repositories such as the Knockout Mouse Project (KOMP), the Mutant Mouse Resource and Research Center (MMRRC) at The Jackson Laboratory, and the European Mouse Mutant Cell Repository (EuMMCR), the Core offers in vitro fertilization (IVF) and embryo transfer services for establishing mutant mouse colonies locally. Furthermore, the core offers blastocyst injection of ES cells carrying mutant alleles for the generation of chimeric mice.
Finally, the Core offers assisted reproductive technology services (ARTS) to preserve and rescue mutant mouse strains. The service portfolio includes embryo and sperm cryopreservation and storage in liquid nitrogen tanks, as well as embryo and sperm resuscitation.